Part:BBa_K3032112:Design

EL222 regulated promoter based on Anderson (0.16)

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 31
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

EL222 DNA binding sequence was carefully inserted into weak promoter’s region of -35 to -10 in order not to affect promoter's activity.

Source

This promoter sequence is design by us. EL222 DNA binding sequence (DOI: 10.1093/nar/gkw548) was inserted into weak promoter’s region of -35 to -10.